| 质粒名称: | pCMV-Neo-Bam APC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 16507 |
| 存储实验室: | Bert Vogelstein |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-Neo-Bam |
| 载体出品公司: | Vogelstein lab (Addgene plasmid# 16440) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6600 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | APC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 9000 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | Bglob-intron-F |
| 3' 测序引物 | Bglob-pA-R (5'-ttttggcagagggaaaaaga-3') |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Neo-Bam APC was a gift from Bert Vogelstein (Addgene plasmid # 16507 ; http://n2t.net/addgene:16507 ; RRID:Addgene_16507) |
| 参考文献部分: | Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW. Science. 1997 Mar 21. 275(5307):1787-90. 10.1126/science.275.5307.1787 PubMed 9065402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Neo-Bam APC 15521 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV-Neo-Bam APC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15521)
AUTHORS Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B,
Kinzler KW
TITLE Activation of beta-catenin-Tcf signaling in colon cancer by
mutations in beta-catenin or APC.
JOURNAL Science. 1997 Mar 21. 275(5307):1787-90.
PUBMED 9065402
REFERENCE 2 (bases 1 to 15521)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15521
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 696..1268
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(10443..10462)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 10508..10563
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(10562..10581)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(10921..10937)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10921..10937)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10934..10956)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10945..10961
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10969..10999)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11014..11035
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11152..11169)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11323..11911)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11403..11422)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12082..12942)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12705..12724
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12943..13047)
/gene="bla"
/label=AmpR promoter
primer_bind 13115..13133
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(13171..13193)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 13293..13312
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 13506..13528
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 13520..13537
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 13521..13537
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 13948..14093
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
CDS 14150..14944
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(14204..14223)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 14814..14833
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(15138..15157)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 15182..15229
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
1321 tcctcgacgg atccgatcga attcccggaa atggggtcca agggtagcca aggatggctg
1381 cagcttcata tgatcagttg ttaaagcaag ttgaggcact gaagatggag aactcaaatc
1441 ttcgacaaga gctagaagat aattccaatc atcttacaaa actggaaact gaggcatcta
1501 atatgaagga agtacttaaa caactacaag gaagtattga agatgaagct atggcttctt
1561 ctggacagat tgatttatta gagcgtctta aagagcttaa cttagatagc agtaatttcc
1621 ctggagtaaa actgcggtca aaaatgtccc tccgttctta tggaagccgg gaaggatctg
1681 tatcaagccg ttctggagag tgcagtcctg ttcctatggg ttcatttcca agaagagggt
1741 ttgtaaatgg aagcagagaa agtactggat atttagaaga acttgagaaa gagaggtcat
1801 tgcttcttgc tgatcttgac aaagaagaaa aggaaaaaga ctggtattac gctcaacttc
1861 agaatctcac taaaagaata gatagtcttc ctttaactga aaatttttcc ttacaaacag
1921 atatgaccag aaggcaattg gaatatgaag caaggcaaat cagagttgcg atggaagaac
1981 aactaggtac ctgccaggat atggaaaaac gagcacagcg aagaatagcc agaattcagc
2041 aaatcgaaaa ggacatactt cgtatacgac agcttttaca gtcccaagca acagaagcag
2101 agaggtcatc tcagaacaag catgaaaccg gctcacatga tgctgagcgg cagaatgaag
2161 gtcaaggagt gggagaaatc aacatggcaa cttctggtaa tggtcagggt tcaactacac
2221 gaatggacca tgaaacagcc agtgttttga gttctagtag cacacactct gcacctcgaa
2281 ggctgacaag tcatctggga accaaggtgg aaatggtgta ttcattgttg tcaatgcttg
2341 gtactcatga taaggatgat atgtcgcgaa ctttgctagc tatgtctagc tcccaagaca
2401 gctgtatatc catgcgacag tctggatgtc ttcctctcct catccagctt ttacatggca
2461 atgacaaaga ctctgtattg ttgggaaatt cccggggcag taaagaggct cgggccaggg
2521 ccagtgcagc actccacaac atcattcact cacagcctga tgacaagaga ggcaggcgtg
2581 aaatccgagt ccttcatctt ttggaacaga tacgcgctta ctgtgaaacc tgttgggagt
2641 ggcaggaagc tcatgaacca ggcatggacc aggacaaaaa tccaatgcca gctcctgttg
2701 aacatcagat ctgtcctgct gtgtgtgttc taatgaaact ttcatttgat gaagagcata
2761 gacatgcaat gaatgaacta gggggactac aggccattgc agaattattg caagtggact
2821 gtgaaatgta tgggcttact aatgaccact acagtattac actaagacga tatgctggaa
2881 tggctttgac aaacttgact tttggagatg tagccaacaa ggctacgcta tgctctatga
2941 aaggctgcat gagagcactt gtggcccaac taaaatctga aagtgaagac ttacagcagg
3001 ttattgcgag tgttttgagg aatttgtctt ggcgagcaga tgtaaatagt aaaaagacgt
3061 tgcgagaagt tggaagtgtg aaagcattga tggaatgtgc tttagaagtt aaaaaggaat
3121 caaccctcaa aagcgtattg agtgccttat ggaatttgtc agcacattgc actgagaata
3181 aagctgatat atgtgctgta gatggtgcac ttgcattttt ggttggcact cttacttacc
3241 ggagccagac aaacacttta gccattattg aaagtggagg tgggatatta cggaatgtgt
3301 ccagcttgat agctacaaat gaggaccaca ggcaaatcct aagagagaac aactgtctac
3361 aaactttatt acaacactta aaatctcata gtttgacaat agtcagtaat gcatgtggaa
3421 ctttgtggaa tctctcagca agaaatccta aagaccagga agcattatgg gacatggggg
3481 cagttagcat gctcaagaac ctcattcatt caaagcacaa aatgattgct atgggaagtg
3541 ctgcagcttt aaggaatctc atggcaaata ggcctgcgaa gtacaaggat gccaatatta
3601 tgtctcctgg ctcaagcttg ccatctcttc atgttaggaa acaaaaagcc ctagaagcag
3661 aattagatgc tcagcactta tcagaaactt ttgacaatat agacaattta agtcccaagg
3721 catctcatcg tagtaagcag agacacaagc aaagtctcta tggtgattat gtttttgaca
3781 ccaatcgaca tgatgataat aggtcagaca attttaatac tggcaacatg actgtccttt
3841 caccatattt gaatactaca gtgttaccca gctcctcttc atcaagagga agcttagata
3901 gttctcgttc tgaaaaagat agaagtttgg agagagaacg cggaattggt ctaggcaact
3961 accatccagc aacagaaaat ccaggaactt cttcaaagcg aggtttgcag atctccacca
4021 ctgcagccca gattgccaaa gtcatggaag aagtgtcagc cattcatacc tctcaggaag
4081 acagaagttc tgggtctacc actgaattac attgtgtgac agatgagaga aatgcactta
4141 gaagaagctc tgctgcccat acacattcaa acacttacaa tttcactaag tcggaaaatt
4201 caaataggac atgttctatg ccttatgcca aattagaata caagagatct tcaaatgata
4261 gtttaaatag tgtcagtagt agtgatggtt atggtaaaag aggtcaaatg aaaccctcga
4321 ttgaatccta ttctgaagat gatgaaagta agttttgcag ttatggtcaa tacccagccg
4381 acctagccca taaaatacat agtgcaaatc atatggatga taatgatgga gaactagata
4441 caccaataaa ttatagtctt aaatattcag atgagcagtt gaactctgga aggcaaagtc
4501 cttcacagaa tgaaagatgg gcaagaccca aacacataat agaagatgaa ataaaacaaa
4561 gtgagcaaag acaatcaagg aatcaaagta caacttatcc tgtttatact gagagcactg
4621 atgataaaca cctcaagttc caaccacatt ttggacagca ggaatgtgtt tctccataca
4681 ggtcacgggg agccaatggt tcagaaacaa atcgagtggg ttctaatcat ggaattaatc
4741 aaaatgtaag ccagtctttg tgtcaagaag atgactatga agatgataag cctaccaatt
4801 atagtgaacg ttactctgaa gaagaacagc atgaagaaga agagagacca acaaattata
4861 gcataaaata taatgaagag aaacgtcatg tggatcagcc tattgattat agtttaaaat
4921 atgccacaga tattccttca tcacagaaac agtcattttc attctcaaag agttcatctg
4981 gacaaagcag taaaaccgaa catatgtctt caagcagtga gaatacgtcc acaccttcat
5041 ctaatgccaa gaggcagaat cagctccatc caagttctgc acagagtaga agtggtcagc
5101 ctcaaaaggc tgccacttgc aaagtttctt ctattaacca agaaacaata cagacttatt
5161 gtgtagaaga tactccaata tgtttttcaa gatgtagttc attatcatct ttgtcatcag
5221 ctgaagatga aataggatgt aatcagacga cacaggaagc agattctgct aataccctgc
5281 aaatagcaga aataaaagaa aagattggaa ctaggtcagc tgaagatcct gtgagcgaag
5341 ttccagcagt gtcacagcac cctagaacca aatccagcag actgcagggt tctagtttat
5401 cttcagaatc agccaggcac aaagctgttg aattttcttc aggagcgaaa tctccctcca
5461 aaagtggtgc tcagacaccc aaaagtccac ctgaacacta tgttcaggag accccactca
5521 tgtttagcag atgtacttct gtcagttcac ttgatagttt tgagagtcgt tcgattgcca
5581 gctccgttca gagtgaacca tgcagtggaa tggtaagtgg cattataagc cccagtgatc
5641 ttccagatag ccctggacaa accatgccac caagcagaag taaaacacct ccaccacctc
5701 ctcaaacagc tcaaaccaag cgagaagtac ctaaaaataa agcacctact gctgaaaaga
5761 gagagagtgg acctaagcaa gctgcagtaa atgctgcagt tcagagggtc caggttcttc
5821 cagatgctga tactttatta cattttgcca cagaaagtac tccagatgga ttttcttgtt
5881 catccagcct gagtgctctg agcctcgatg agccatttat acagaaagat gtggaattaa
5941 gaataatgcc tccagttcag gaaaatgaca atgggaatga aacagaatca gagcagccta
6001 aagaatcaaa tgaaaaccaa gagaaagagg cagaaaaaac tattgattct gaaaaggacc
6061 tattagatga ttcagatgat gatgatattg aaatactaga agaatgtatt atttctgcca
6121 tgccaacaaa gtcatcacgt aaagcaaaaa agccagccca gactgcttca aaattacctc
6181 cacctgtggc aaggaaacca agtcagctgc ctgtgtacaa acttctacca tcacaaaaca
6241 ggttgcaacc ccaaaagcat gttagtttta caccggggga tgatatgcca cgggtgtatt
6301 gtgttgaagg gacacctata aacttttcca cagctacatc tctaagtgat ctaacaatcg
6361 aatcccctcc aaatgagtta gctgctggag aaggagttag aggaggagca cagtcaggtg
6421 aatttgaaaa acgagatacc attcctacag aaggcagaag tacagatgag gctcaaggag
6481 gaaaaacctc atctgtaacc atacctgaat tggatgacaa taaagcagag gaaggtgata
6541 ttcttgcaga atgcattaat tctgctatgc ccaaagggaa aagtcacaag cctttccgtg
6601 tgaaaaagat aatggaccag gtccagcaag catctgcgtc gtcttctgca cccaacaaaa
6661 atcagttaga tggtaagaaa aagaaaccaa cttcaccagt aaaacctata ccacaaaata
6721 ctgaatatag gacacgtgta agaaaaaatg cagactcaaa aaataattta aatgctgaga
6781 gagttttctc agacaacaaa gattcaaaga aacagaattt gaaaaataat tccaaggact
6841 tcaatgataa gctcccaaat aatgaagata gagtcagagg aagttttgct tttgattcac
6901 ctcatcatta cacgcctatt gaaggaactc cttactgttt ttcacgaaat gattctttga
6961 gttctctaga ttttgatgat gatgatgttg acctttccag ggaaaaggct gaattaagaa
7021 aggcaaaaga aaataaggaa tcagaggcta aagttaccag ccacacagaa ctaacctcca
7081 accaacaatc agctaataag acacaagcta ttgcaaagca gccaataaat cgaggtcagc
7141 ctaaacccat acttcagaaa caatccactt ttccccagtc atccaaagac ataccagaca
7201 gaggggcagc aactgatgaa aagttacaga attttgctat tgaaaatact ccagtttgct
7261 tttctcataa ttcctctctg agttctctca gtgacattga ccaagaaaac aacaataaag
7321 aaaatgaacc tatcaaagag actgagcccc ctgactcaca gggagaacca agtaaacctc
7381 aagcatcagg ctatgctcct aaatcatttc atgttgaaga taccccagtt tgtttctcaa
7441 gaaacagttc tctcagttct cttagtattg actctgaaga tgacctgttg caggaatgta
7501 taagctccgc aatgccaaaa aagaaaaagc cttcaagact caagggtgat aatgaaaaac
7561 atagtcccag aaatatgggt ggcatattag gtgaagatct gacacttgat ttgaaagata
7621 tacagagacc agattcagaa catggtctat cccctgattc agaaaatttt gattggaaag
7681 ctattcagga aggtgcaaat tccatagtaa gtagtttaca tcaagctgct gctgctgcat
7741 gtttatctag acaagcttcg tctgattcag attccatcct ttccctgaaa tcaggaatct
7801 ctctgggatc accatttcat cttacacctg atcaagaaga aaaacccttt acaagtaata
7861 aaggcccacg aattctaaaa ccaggggaga aaagtacatt ggaaactaaa aagatagaat
7921 ctgaaagtaa aggaatcaaa ggaggaaaaa aagtttataa aagtttgatt actggaaaag
7981 ttcgatctaa ttcagaaatt tcaggccaaa tgaaacagcc ccttcaagca aacatgcctt
8041 caatctctcg aggcaggaca atgattcata ttccaggagt tcgaaatagc tcctcaagta
8101 caagtcctgt ttctaaaaaa ggcccacccc ttaagactcc agcctccaaa agccctagtg
8161 aaggtcaaac agccaccact tctcctagag gagccaagcc atctgtgaaa tcagaattaa
8221 gccctgttgc caggcagaca tcccaaatag gtgggtcaag taaagcacct tctagatcag
8281 gatctagaga ttcgacccct tcaagacctg cccagcaacc attaagtaga cctatacagt
8341 ctcctggccg aaactcaatt tcccctggta gaaatggaat aagtcctcct aacaaattat
8401 ctcaacttcc aaggacatca tcccctagta ctgcttcaac taagtcctca ggttctggaa
8461 aaatgtcata tacatctcca ggtagacaga tgagccaaca gaaccttacc aaacaaacag
8521 gtttatccaa gaatgccagt agtattccaa gaagtgagtc tgcctccaaa ggactaaatc
8581 agatgaataa tggtaatgga gccaataaaa aggtagaact ttctagaatg tcttcaacta
8641 aatcaagtgg aagtgaatct gatagatcag aaagacctgt attagtacgc cagtcaactt
8701 tcatcaaaga agctccaagc ccaaccttaa gaagaaaatt ggaggaatct gcttcatttg
8761 aatctctttc tccatcatct agaccagctt ctcccactag gtcccaggca caaactccag
8821 ttttaagtcc ttcccttcct gatatgtctc tatccacaca ttcgtctgtt caggctggtg
8881 gatggcgaaa actcccacct aatctcagtc ccactataga gtataatgat ggaagaccag
8941 caaagcgcca tgatattgca cggtctcatt ctgaaagtcc ttctagactt ccaatcaata
9001 ggtcaggaac ctggaaacgt gagcacagca aacattcatc atcccttcct cgagtaagca
9061 cttggagaag aactggaagt tcatcttcaa ttctttctgc ttcatcagaa tccagtgaaa
9121 aagcaaaaag tgaggatgaa aaacatgtga actctatttc aggaaccaaa caaagtaaag
9181 aaaaccaagt atccgcaaaa ggaacatgga gaaaaataaa agaaaatgaa ttttctccca
9241 caaatagtac ttctcagacc gtttcctcag gtgctacaaa tggtgctgaa tcaaagactc
9301 taatttatca aatggcacct gctgtttcta aaacagagga tgtttgggtg agaattgagg
9361 actgtcccat taacaatcct agatctggaa gatctcccac aggtaatact cccccggtga
9421 ttgacagtgt ttcagaaaag gcaaatccaa acattaaaga ttcaaaagat aatcaggcaa
9481 aacaaaatgt gggtaatggc agtgttccca tgcgtaccgt gggtttggaa aatcgcctga
9541 actcctttat tcaggtggat gcccctgacc aaaaaggaac tgagataaaa ccaggacaaa
9601 ataatcctgt ccctgtatca gagactaatg aaagttctat agtggaacgt accccattca
9661 gttctagcag ctcaagcaaa cacagttcac ctagtgggac tgttgctgcc agagtgactc
9721 cttttaatta caacccaagc cctaggaaaa gcagcgcaga tagcacttca gctcggccat
9781 ctcagatccc aactccagtg aataacaaca caaagaagcg agattccaaa actgacagca
9841 cagaatccag tggaacccaa agtcctaagc gccattctgg gtcttacctt gtgacatctg
9901 tttaaaagag aggaagaatg aaactaagaa aattctatgt taattacaac tgctatatag
9961 acattttgtt tcaaatgaaa ctttaaaaga ctgaaaaatt ttgtaaatag gtttgattct
10021 tgttagaggg tttttgttct cgaagccata tttgatagta tactttgtct tcactggtct
10081 tattttggga ggcactcttg atggttagga aaaaaatagt aaagccaagt atgtttgtac
10141 agtatgtttt acatgtattt aaagtagcat cccatcccaa cttcctttaa ttattgcttg
10201 tcttaaaata atgaacacta cagatagaaa atatgatata ttgctgttat caatcatttc
10261 tagattataa actgactaaa cttacatcag ggaaaaattg gtatttatgc aaaaaaaaat
10321 gtttttgtgg aattcctgca gcccggggga tccgtcgagg aattcactcc tcaggtgcag
10381 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
10441 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
10501 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
10561 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
10621 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
10681 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
10741 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
10801 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
10861 atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat
10921 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
10981 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
11041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
11101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
11161 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
11221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
11281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
11341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
11401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
11461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
11521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
11581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
11641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
11701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
11761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
11821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
11881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
11941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
12001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
12061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
12121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
12181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
12241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
12301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
12361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
12421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
12481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
12541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
12601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
12661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
12721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
12781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
12841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
12901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
12961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
13021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
13081 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
13141 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
13201 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
13261 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
13321 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
13381 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
13441 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
13501 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttcacg ctgccgcaag
13561 cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
13621 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
13681 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
13741 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
13801 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
13861 agatcctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
13921 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
13981 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
14041 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
14101 aacagcgtgc cgcagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
14161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
14221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
14281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
14341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
14401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
14461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
14521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
14581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
14641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
14701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
14761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
14821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
14881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
14941 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
15001 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
15061 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg
15121 agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
15181 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
15241 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
15301 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
15361 gcagccaacg tcggggcggc aagccctgcc atagccacgg gccccgtggg ttagggacgg
15421 cggatcgcgg ccctctagag agcttggccc attgcatacg ttgtatccat atcataatat
15481 gtacatttat attggctcat gtccaacatt accgccatgt t
//
